Buy now0.8 nm Undecagold Labeling Reagents


Updated: January 1, 2020

Ultra-small --The best choice for Cryo-EM.

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Undecagold (Au11) is smaller than Nanogold®, with a core of 11 gold atoms only 0.8 nm in diameter. It is ideal for ultra-high-resolution EM work such as cryo-EM, scanning transmission electron microscopy (STEM), or for resolving elements of large structures by TEM in conjunction with image processing. Undecagold has been used to see the biotin binding sites on avidin to 1 nm resolution by electron microscopy. It is prepared in a form with one reactive arm for cross-linking to a specific site on a target molecule, and is available with different reactivities for labeling different sites.

  • Ultra small 0.8 nm gold core.
  • Use to prepare the smallest possible gold probes.
  • Highest possible resolution.
  • Site specific covalent labeling with choice of reactivities.

Note: Undecagold is perfect for labeling in high-resolution applications like cryo-EM, but single Undecagold clusters are not routinely visualized directly in the TEM. For standard TEM, Undecagold may be seen upon image processing of protein helices and crystals, or visualized en masse if there is a bulk deposition such as staining of an organelle. Also, Undecagold develops more slowly and with less final developer deposition during silver enhancement or gold enhancement than with our larger, 1.4 nm Nanogold®. Therefore, for many applications we recommend 1.4 nm Nanogold®.

Undecagold Structure (27k)
Structure of undecagold showing the [Au11] core, coordinated ligands and peripheral reactive group (maleimide).


Buy nowApplications

 

Undecagold (Au11) Labeling Reagents

Buy nowMonomaleimido-Undecagold

The simple-to-use activated Maleimido-undecagold reagent is used for labeling thiols with undecagold. It will specifically label free sulfhydryls in proteins, antibodies, peptides, substrates or other molecules containing a free thiol. For use, it is simply dissolved in water and mixed with the material to be labeled for a few hours. Undecagold conjugates are then isolated by gel filtration.

Applications:

  • Create your own small gold probes by linking Au11 to your primary antibody Fab' fragments.
  • Label proteins directly for high resolution, site specific identification.
  • Label peptides containing cysteine.

 


Buy nowMono-Sulfo-NHS-Undecagold

This reagent is used to covalently attach undecagold to primary amines.

Applications:

  • Label proteins which do not contain a cysteine.
  • Label peptides at the N-terminal.
  • Undecagold-label small molecules as inhibitors or substrate analogs for probing transition state structures.

 


Buy nowClick Undecagold

Click chemistry is the reaction between an azide and an alkyne or similar group. It is a bioorthogonal reaction (i.e. it does not occur naturally) which lets let you label selectively in living cells, tissues and even animals without cross-reactivity. Click Undecagold reagents are stable, biocompatible, and well tolerated by living cells. They provide ultra-high resolution, putting the Undecagold right on the target site: locate targets at macromolecular resolution.

Both reactivities are available:

  • Mono-Azido Undecagold reacts with alkynes and related reagents; may be used for some copper-free click reactions.
  • Mono-Alkyne Undecagold reacts with azides.

Applications:

  • Label dynamic processes as they happen in living cells or tissues - Click reagents will not hydrolyze or degrade.
  • Label components in living cells: Ignore endogenous species like biotin or cross-reactive proteins.
  • High-resolution labeling of subunits or molecular sites: just incorporate the corresponding reactive azide or alkyne - no antibody or probe required.
  • Multiplex your labeling: Add a click reaction to detect an additional target if there is no suitable antibody or probe.
Undecagold Click labeling reactions

 


Buy nowMonoamino Undecagold

Use this reagent to label carbohydrates and glycoproteins, or activate with a heterobifunctional cross-linking reagent for different reactivity.

 


Buy nowCharged Undecagold

Positively and negatively charged undecagold may be used as ionic labels, to stain biological structures with opposite charges. Positive undecagold has multiple amines on its surface and may therefore stain negatively charged features; negatively charged undecagold has multiple carboxyl groups, and will stain features containing many positively ionizing groups.

 


Buy nowNon-Functionalized Undecagold

Non-fuctionalized undecagold is an inert, stable form of the undecagold particle with no reactive chemical functionalities or specific affinity properties.

Applications:

  • As a size standard for calibration in high-resolution electron microscopy.
  • Sizing pores (see: Schwartz, M. P., and Matouschek, A.: The dimensions of the protein import channels in the outer and inner mitochondrial membranes. Proc. Natl. Acad. Sci. USA, 96, 13086-13090 (1999)). [Complete references]
  • For tracing contiguous interior spaces in cells and tissues.

 


Custom Undecagold Conjugates

Nanoprobes does not stock undecagold conjugates, but we will be glad to prepare them to order as a custom synthesis. Let us know your needs, and we'll give you a quote: Use our online form, e-mail us at [email protected], or call us at 877-447-6266 (US toll-free number) or (631) 205-9490. We look forward to working with you!

 

Price List:
Undecagold Reagents
Custom undecagold conjugates are also available.
Monomaleimido Undecagold
#2030-50NMOL
Monomaleimido Undecagold
Au11 cluster with single maleimide group for selectively labeling thiols (-SH). May be used to label primary Fab' antibody fragments, cysteine groups on proteins, or other sulfhydryl containing compounds. Reagent and procedure to label 300 mg Fab'. 50 nmol
$509
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#2030A-5X10NMOL
Monomaleimido Undecagold
in 5 aliquots

Same total quantity as 2030-50 nmol, but provided in 5 separate tubes. Since the malieide moiety is unstable once dissolved, separate aliquots are preferable if several smaller-quantity labeling reactions are planned at different times.

50 nmol
in 5 aliquots
$563

Qty:

Code (Optional)

#2030S-10NMOL
Monomaleimido Undecagold introductory size
Same as 2030-50 nmol, but packaged in an introductory size for smaller labeling experiments. 10 nmol
$148
Qty:

Code (Optional)

Monoamino Undecagold
#2031-50NMOL
Monoamino Undecagold
Au11 cluster with a single primary amine. May be used for labeling the carbohydrate moiety of glycoproteins or other uses. 50 nmol
$349
Qty:

Code (Optional)

#2031A-5X10NMOL
Monoamino Undecagold
in 5 aliquots

Same total quantity as 2031-50 nmol, but provided in 5 separate tubes. Preferable if several smaller-quantity labeling reactions are planned at different times.
50 nmol
in 5 aliquots
$399
Qty:

Code (Optional)

#2031S-10NMOL
Monoamino Undecagold introductory size
Same as 2031-50 nmol, but packaged in an introductory size for smaller labeling experiments. 10 nmol
$103
Qty:

Code (Optional)

Mono-Sulfo-NHS-Undecagold
#2045-50NMOL
Mono-Sulfo-NHS-Undecagold
Undecagold with a single N-hydroxy-sulfosuccinimidyl group. Used for labeling primary amines. 50 nmol
$509
Qty:

Code (Optional)

#2045A-5X10NMOL
Mono-Sulfo- NHS-Undecagold
in 5 aliquots

Same total quantity as 2045-50 nmol, but provided in 5 separate tubes. Preferable if several smaller-quantity labeling reactions are planned at different times.
50 nmol
in 5 aliquots
$563
Qty:

Code (Optional)

#2045S-10NMOL
Mono-Sulfo-NHS-Undecagold introductory size
Same as 2045-50 nmol, but packaged in an introductory size for smaller labeling experiments. 10 nmol
$148
Qty:

Code (Optional)

Click Undecagold: Mono-Azido-Undecagold
#2046-50NMOL
Mono-Azido-Undecagold
Undecagold with a single azide group. Used for click labeling of alkynes and related compounds. 50 nmol
$509
Qty:

Code (Optional)

#2046-5X10NMOL
Mono-Azido-Undecagold
in 5 aliquots

Same total quantity as 2046-50 nmol, but provided in 5 separate tubes. Convenient if several smaller-quantity labeling reactions are planned at different times.
50 nmol
in 5 aliquots
$563
Qty:

Code (Optional)

#2046-10NMOL
Mono-Azido-Undecagold introductory size
Same as 2046-50 nmol, but packaged in an introductory size for smaller labeling experiments. 10 nmol
$148
Qty:

Code (Optional)

Click Undecagold: Mono-Alkyne-Undecagold
#2047-50NMOL
Mono-Alkyne-Undecagold
Undecagold with a single akyne group. Used for click labeling of azides. 50 nmol
$509
Qty:

Code (Optional)

#2047-5X10NMOL
Mono-Alkyne-Undecagold
in 5 aliquots

Same total quantity as 2047-50 nmol, but provided in 5 separate tubes. Convenient if several smaller-quantity labeling reactions are planned at different times.
50 nmol
in 5 aliquots
$563
Qty:

Code (Optional)

#2047-10NMOL
Mono-Alkyne-Undecagold introductory size
Same as 2047-50 nmol, but packaged in an introductory size for smaller labeling experiments. 10 nmol
$148
Qty:

Code (Optional)

Charged Undecagold
#2043-50NMOL
Positively Charged Undecagold
Undecagold with a net positive charge; contains amino groups. 50 nmol
$357
Qty:

Code (Optional)

#2044-50NMOL
Negatively Charged Undecagold
Undecagold with a net negative charge; contains carboxyl groups. 50 nmol
$357
Qty:

Code (Optional)

Non-Functionalized Undecagold
#2060-50NMOL
Undecagold Particles, Non-Functionalized
Undecagold particles only, lyophilized; non-reactive form. 50 nmol
$238
Qty:

Code (Optional)

Undecagold Conjugates
Custom Undecagold Conjugates Nanoprobes does not stock undecagold conjugates, but we will be happy to prepare them to order as a custom synthesis. Contact us for a quote
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